Sterility:Article Title: Lipidomics of oxidized polyunsaturated fatty acids
Article Snippet: RC DC protein assay kit II using bovine serum albumin (BSA) as protein standard (Cat. No. 500-0122) was from Bio-Rad Laboratories Ltd (Hemel Hempstead, UK). .. Ninety-six-well microplates (PS, sterile; Cat. No. 655161) were from Greiner Bio-One Ltd (Stonehouse, UK). .. The following analytical-grade standards were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA): 15-deoxy-Δ 12,14 -PGJ 2 (Cat. No. 18570), PGJ 2 (Cat. No. 18500), Δ 12 -PGJ 2 (Cat. No. 18550), 15-keto-PGE 2 (Cat. No. 14720), PGE 3 (Cat. No. 14990), PGD 3 (Cat. No. 12990), PGE 2 (Cat. No. 14010), PGD 2 (Cat. No. 12010), PGF 2α (Cat. No. 16010), PGE 1 (Cat. No. 13010), PGD 1 (Cat. No. 12000), PGF 1α (Cat. No. 15010), TXB 3 (Cat. No. 19990), TXB 2 (Cat. No. 19030), 6-keto-PGF 1α (Cat. No. 15210), 13,14-dihydro-PGF 2α (Cat. No. 16660), 13,14-dihydro-PGE 1 (Cat. No. 13610), 13,14-dihydro-15-keto-PGE 2 (Cat. No. 14650), 13,14-dihydro-15-keto-PGF 2α (Cat. No. 16670), 13,14-dihydro-15-keto-PGE 1 (Cat. No. 13650), 13,14-dihydro-15-keto-PGF 1α (Cat. No. 15670), PGB 2 - d 4 (Cat. No. 311210), ±9-HODE (Cat. No. 38400), ±13-HODE (Cat. No. 38600), ±5-HEPE (Cat. No. 32200), ±8-HEPE (Cat. No. 32340), ±9-HEPE (Cat. No. 32400), ±11-HEPE (Cat. No. 32500), ±12-HEPE (Cat. No. 32540), ±15-HEPE (Cat. No. 32700), ±18-HEPE (Cat. No. 32840), ±5-HETE (Cat. No. 34210), ±8-HETE (Cat. No. 34340), ±9-HETE (Cat. No. 34400), ±11-HETE (Cat. No. 34500), ±12-HETE (Cat. No. 34550), ±15-HETE (Cat. No. 34700), ±10-HDHA (Cat. No. 33400; ±13-HDHA (Cat. No. 33500), ±14-HDHA (Cat. No. 33550), ±17-HDHA (Cat. No. 33650), ±20-HDHA (Cat. No. 33750), 15( S )-HETrE (Cat. No. 36720), LTB 4 (Cat. No. 20110), RvD 1 (Cat. No. 10012554), 5( S ),6( R ),15( S )-LXA 4 (Cat. No. 90410), 5( S ),6( R ),15( R )-LXA 4 (15-epi-LXA 4 ; Cat. No. 90415), 5-oxo-ETE (Cat. No. 90415), ±5(6)-EET (Cat. No. 50211), ±8(9)-EET (Cat. No. 50351), ±11(12)-EET (Cat. No. 50511), ±14(15)-EET (Cat. No. 50651), ±5,6-DHET (Cat. No. 51211), ±8,9-DHET (Cat. No. 51351), ±11,12-DHET (Cat. No. 51511), ±14,15-DHET (Cat. No. 51651), and 12( S )-HETE- d 8 (Cat. No. 334570).
Sandwich ELISA:Article Title: Estimation of polyclonal IgG4 hybrids in normal human serum
Article Snippet: Chromatography was performed using a GILSON HPLC system with UV detection, controlled by clarity software. .. Sandwich ELISA analysis Ninety-six-well microplates (Greiner Bio-One, Stonehouse, UK) were coated with 100 μl per well of either anti-total κ light-chain polyclonal antibody or anti-total λ light-chain polyclonal antibody (The Binding Site, Birmingham, UK), both at 5 μg/ml in PBS-azide (25 m m NaH 2 PO 4 , 150 m m NaCl, 0·1% NaN 3 ; pH 7). ..
Binding Assay:Article Title: Estimation of polyclonal IgG4 hybrids in normal human serum
Article Snippet: Chromatography was performed using a GILSON HPLC system with UV detection, controlled by clarity software. .. Sandwich ELISA analysis Ninety-six-well microplates (Greiner Bio-One, Stonehouse, UK) were coated with 100 μl per well of either anti-total κ light-chain polyclonal antibody or anti-total λ light-chain polyclonal antibody (The Binding Site, Birmingham, UK), both at 5 μg/ml in PBS-azide (25 m m NaH 2 PO 4 , 150 m m NaCl, 0·1% NaN 3 ; pH 7). ..
Article Title: Generation, characterization, and application of caprine herpesvirus 1 secreted glycoprotein D.
Article Snippet: .. Samples collection and ELISA procedure Ninety-six-well microplates (Microlon High Binding, Greiner Bio-One, Kremsmünster, Austria) were coated overnight at 4°C with 50 ng/well of Sec-gD protein supernatant diluted in 0.1 M carbonate/bicarbonate buffer at pH 9.6. .. After blocking with 1% BSA (Sigma Aldrich by Merck, Rome, Italy), goat serum samples at different twofold dilutions (1/10, 1/20, 1/40, 1/80, 1/160, 1/320, 1/640, and 1/1,280) were incubated for 1 h at room temperature.
Enzyme-linked Immunosorbent Assay:Article Title: Generation, characterization, and application of caprine herpesvirus 1 secreted glycoprotein D.
Article Snippet: .. Samples collection and ELISA procedure Ninety-six-well microplates (Microlon High Binding, Greiner Bio-One, Kremsmünster, Austria) were coated overnight at 4°C with 50 ng/well of Sec-gD protein supernatant diluted in 0.1 M carbonate/bicarbonate buffer at pH 9.6. .. After blocking with 1% BSA (Sigma Aldrich by Merck, Rome, Italy), goat serum samples at different twofold dilutions (1/10, 1/20, 1/40, 1/80, 1/160, 1/320, 1/640, and 1/1,280) were incubated for 1 h at room temperature.
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